Microbiology
BS 2314, Section A
Spring Semester 2008
Lecture and lab: TTH 8:00 - 10:45 SC101 (lecture) and SC111 (lab)
Course description: The study of the taxonomy, morphology, and physiology of microorganisms, emphasizing: 1) their relation to medicine, industry, agriculture; and 2) basic lab technique.
Prerequisites: Biological Science (BS1114) will be a prerequisite for Microbiology beginning with the 2008/2009 catalogue. We strongly recommend that you have completed biological science before taking this course.
Textbooks: Prescott,
Harley, Klein’s Microbiology. J. Willey, L. Sherwood, and C. Woolverton. Seventh edition. WCB/McGraw-Hill Press,
Rationale:
This course integrates an introduction to microbiology with an emphasis on the important skills of: 1) critical thinking; 2) scientific writing; and 3) public presentation. Although the specific focus is on microorganisms, the course incorporates a thorough review of important subjects (e.g., cellular respiration, enzyme activity, basic genetics, use of microbes in genetic study and biotechnology). Microbiology is highly relevant to both scientists and the general public. For example, microbes are used in genetic studies, for industrial purposes, and for medical research. This course seeks to provide students with an understanding of important scientific concepts, an ability to think critically, and an understanding of the importance of microbiology to society in general. Additionally, this course seeks to provide an appreciation of the importance of the natural world.
Course objectives:
1. to develop a sufficient background for those students who wish to study more advanced topics
2. to provide familiarity with basic microbiological laboratory techniques
3. to aid the development of the ability to think scientifically and to evaluate information critically
4. to provide an understanding of the role of microbes in disease transmission and prevention
5. to provide familiarity with important laboratory safety guidelines
6. to improve public speaking and scientific writing skills through lab reports, presentations, and discussions
Institutional Goals: This course will address the six institutional goals (found on page 9 of the catalogue) as follows.
1. Institutional goal 1 (“To produce students who demonstrate an understanding of the Judeo-Christian heritage and Christian worldview”) will be addressed by objectives 1, 2, and 4. Students will learn aspects of the historical development of microbiology, consider current ethical issues, and demonstrate their perspectives through exams and reports.
2. Institutional goal 2 (“To produce students who demonstrate the ability to communicate effectively across the curriculum”) will be addressed by primarily by objective 6, but will also be addressed by exam questions, homework assignments, and class discussions.
3. Institutional goal 3 (“To produce students who demonstrate the ability to reflect critically upon the world, the environment, society, and self”) will be addressed within the context of class discussions, through exam questions, and through lab reports. In particular, objective 3 addresses this goal.
4. Institutional goal 4 (“To produce students who demonstrate the ability to apply the principles of the scientific method to become more effective problem solvers”) will be addressed through problem solving during class, homework, and exams (reflected in objective 3). This goal will also be addressed through the independent research projects that students will complete during lab.
5. Institutional goal 5 (“To produce students who demonstrate an appreciation for a healthy lifestyle that will promote lifelong heath and fitness”) will be addressed through discussion of the biological bases of health and disease (encompassed within objectives 1, 2, and 4).
6. Institutional goal 6 (“To produce students who demonstrate a competence in at least one particular body of knowledge”) will be addressed through strengthening student knowledge of biology, and of important general scientific concepts, through all aspects of the course.
Course Requirements:
Attendance and completion of all exams is mandatory. Students are accountable for all class assignments, class announcements, handouts, and information provided in lecture. After three lecture or lab absences, a student’s course grade will be reduced by 5% for each additional absence. If more than six lectures or labs are missed, the student will be asked to drop the course or receive a failing grade. Failure to clean up a work station following lab will result in a loss of 5 points for every student working at that station. Please arrive on time to lab! You will not be allowed to stay late to work because you have arrived late and need extra time.
If you must miss an exam or lab, then you must contact me as quickly as possible. It is always better to contact me before rather than after an absence if you know in advance that you must miss class (if you notify me in advance, it will be easier to receive approval for a makeup). Please provide a substantive excuse (e.g., a doctor’s note). Do not assume that you will be able to make up a lab, lab practical, or exam. Additionally, please do not ask to postpone an exam on the day of the exam. If you have other exams on the same day, bring it to my attention before the exam date. In particular, note that some labs may be difficult or impossible to make up.
Your grade will be lowered by 5% of the total possible number of points for each day that an assignment is late. If an assignment is due in a particular lab or class, that means that it is due at the beginning of the lab or class and you will lose points if you turn it in during or after the class on the same day.
Students are expected and required to uphold the highest standards of academic honesty in this and all courses. Students should be familiar with the College's policies concerning academic integrity Catalog, page 46; Student Handbook, page 24, section VII). Students requiring any clarification of these policies should consult their academic advisor or the Office of Academic Affairs. Student papers will be submitted to Turnitin.com and will be penalized heavily for plagiarism. Substantial plagiarism will result in a grade of 0 and possible additional penalties. If you are uncertain about what constitutes plagiarism and want assistance, please speak with Dr. Paterson.
Evaluation:
Lecture Exam I 50 pts
Lecture Exam II 100 pts
Lecture Exam III 100 pts
Final Exam 200 pts
Presentations 50 pts
Quizzes 50 pts
Lab notebook 50 pts
Lab reports 50 pts
Lab Practical I 25 pts
Lab Practical II 25 pts
Total 700 pts
There will be short quizzes at the beginning of each lab. In addition, you are required to keep a lab notebook in which you take notes on each lab. Lab reports will be short papers written in scientific format. Your presentation will consist of a short overview of a microbial taxon, such as those outlined in your text.
Instructor: Dr. Ann V. Paterson
Phone: 870-759-4171
E-mail:
Office hours: I will be in my office from: 9:00 – 11:00 am on Mondays, Wednesdays, and Fridays; from 2:00 – 2:30 pm on Mondays; from 2:00 – 2:30 pm on Tuesdays; from 12:00 pm – 1:00 pm on Wednesdays; and from 1:00 – 3:00 pm on Thursdays. Please stop by, call, or e-mail if you would like to schedule an appointment at a different time. My class schedule is posted on my office door in case you would like to try to find me in a class or lab. If you need to contact me, e-mail is generally the fastest method.
Microbiology Syllabus
Note: this syllabus will change during the semester. You are responsible for changes announced in lecture (e.g., changes in exam dates).
Week Date Lecture
Topic Text
Assignment*
1 Jan. 8 Introduction Chapter 1
Brief history and relevance of microbiology
Jan. 10 Microscopy Chapter 2
Prokaryotic cell structure and function Chapter 3
2 Jan. 15 Eukaryotic cell structure and function Chapter 4
Last day to add a class
Jan. 17 Mitosis and meiosis
3 Jan. 22 Overview of prokaryotic and eukaryotic cells
Jan. 24 Identifying unknowns
Nutritional requirements Chapter 5
4 Jan. 29 Growth patterns Chapter 6
Jan. 31 Review/catch-up
5 Feb. 5 Lecture exam I
Feb. 7 Culturing microorganisms Chapter 6
Control of microorganisms Chapter 7
6 Feb. 12 Why we use detergents and soap Chapter 7
Introduction to metabolism Chapter 8
Feb. 14 Enzymes Chapter 8
Metabolism (general) Chapter 8
Chapter 9
7 Feb. 19 Metabolic pathways (cellular respiration) Chapter 9
Feb. 21 Metabolic pathways (photosynthesis) Chapter 9
8 Feb. 26 Review/catch-up
Feb. 28 Lecture
exam II
9 Mar. 4 Biosynthesis Chapter 10
Mar. 6 Introduction/review of genetic concepts Chapter 11
Synthesis of nucleic acids Chapter 11
10 Mar. 11 Spring break
Mar. 13 Spring break
11 Mar. 18 Synthesis of nucleic acids, cont. Chapter 11
Chapter 12
Mar. 20 Protein synthesis Chapter 11
Mutations and repair Chapter 11
12 Mar. 25 Microbial genetics Chapters 13, 14, 15
Mar. 27 Microbial genetics Chapters 13, 14, 15
13 Apr. 1 Viruses, microbial taxonomy Chapters 16-19
Apr. 3 Review/catch-up
14 Apr. 8 Lecture exam III
Apr. 10 Symbiotic associations Chapter 28
15 Apr. 15 Student presentations
Apr. 17 Immunology, diseases Chapters 31-40
16 Apr. 22 Diseases Chapters 38-70
Environmental microbiology, industrial Chapters 29-30, 41-42
microbiology, microbiology of food
Apr. 24 Review/catch-up
Apr. 25 – Apr. 30 Final exams
* note that detailed text assignments will be given in lecture.
Microbiology Lab Syllabus
Week Date Lab
Exercise
2 Jan. 15 Introduction, general laboratory safety
Light microscope
Identifying cocci, bacilli, and spirilla
Slides of representative microbes
3 Jan. 22 Microscopic measurement
Introduction to sterile technique
4 Jan. 29 Staining techniques
Identifying unknowns
5 Feb. 5 Identifying unknowns
6 Feb. 12 Media preparation and culture techniques
7 Feb. 19 Spread and streak plate techniques
Isolation and maintenance of pure cultures
8 Feb. 26 Lab practical I
9 Mar. 4 Starch and casein hydrolysis
10 Mar. 11 Spring break
11 Mar. 18 Effects of antibiotics
12 Mar. 25 Determination of bacterial numbers
Effects of disinfectants, part 1
13 Apr. 1 Effects of disinfectants, part 2
Design an experiment – preparation
First lab report due
14 Apr. 8 Run your experiments
15 Apr. 15 Present results
Second lab report due
16 Apr. 22 Lab practical II
Apr. 25 – Apr. 30 Final exams